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KMID : 0370220090530030114
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Yakhak Hoeji
2009 Volume.53 No. 3 p.114 ~ p.118
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Emulsifying Character of -Glucosidase Inhibitor Produced from Bacillus lentimorbus B-6
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Yang Young-Joon
Kim Kyoung-Ja
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Abstract
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Bioemulsifiers are those chemicals which are produced from microorganisms but which have both hydrophilic and hydrophobic groups. -Glucosidase inhibitor (-GI) produced from Bacillus lentimorbus B-6 (B-6) showed bioemulsifying activity. But -glucosidase inhibitor produced from B-6 didn’t show emulsifying activity. -GI was purified from supernatant of B-6 grown in minimal culture medium containing glucose and sodium glutamate by Sephadex G-100 column chromatography and isolated from -GI by dialysis against water. Toluene was determined as the best substrate for emulsifying activity of -GI. -GI showed thermostability at for 15 min, high salt tolerance up to 32% NaCl and wide range of pH-stability at pH . Emulsifying character of -GI can be useful for the liposome formation for the treatment of diabetes mellitus.
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KEYWORD
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-glucosidase inhibitor, Bacillus lentimorbus, bioemulsifier
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